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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which ends up in chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants have been described but thorough nucleotide sequence details just isn't nevertheless readily available.
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Blocking peptides are peptides that bind particularly for the goal antibody and block antibody binding. These peptide usually incorporates the epitope recognized with the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines even further attainable optimization of mobile and immunoassay parameters to be sure the best possible benefits are...
Antibodies sure to the blocking peptide no longer bind on the epitope about the goal protein. By comparing the staining in the blocked antibody vs . the antibody alone, one can see which staining is precise.
Focus on Info Cyclin E belongs to your highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features being a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates in the G1-S stage boundary and is particularly degraded as cells development by S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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